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BI452 Biochemistry Principles and Experimental Design Assignment Example NUI Galway Ireland

In BI452 Biochemistry Principles and Experimental Design course, students will learn about the principles of biochemistry and experimental design. The course will cover topics such as the structure and function of biomolecules, enzyme kinetics, metabolism, and biochemical regulation. Students will also learn how to design and carry out experiments to test hypotheses about biochemical processes.

Throughout the course, students will have the opportunity to apply what they learn in practical exercises, including designing and carrying out experiments in the laboratory. The course will cover the basic concepts of chemistry and physics as they relate to the study of biological systems. The course will also cover the basic principles of experimental design and data analysis.

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In this course, there are many types of assignments given to students like group projects, individual assignments, continuous assessments, reports, business plans, business proposals, executive summaries, and other solutions given by us.

In this section, we are describing some assigned activities. These are:

Assignment Task 1: Define and discuss key elements of experimental design.

When designing an experiment, it is important to consider the following elements:

  • The question or hypothesis that you are testing: The question or hypothesis that you are testing is the most important element of your experiment. It is essential to have a clear idea of what you are trying to learn before you start designing your experiment. Once you have a hypothesis, you can design an experiment that will best answer that question.
  • The experimental design that will best answer your question: The experimental design that you choose will depend on the question you are trying to answer. There are many different types of experimental designs, and each has its strengths and weaknesses. It is important to choose a design that will allow you to collect the type of data you need to answer your question.
  • The type of data you will collect and how you will analyze it: The type of data you collect and how you analyze it will also depend on the question you are trying to answer. Some types of data are more useful than others for answering specific questions. It is important to have a plan for analyzing your data before you start collecting it.
  • The controls that you will use in your experiment: The controls that you use in your experiment are also important. A control is a variable that is kept the same in all of the experiments in a study. By keeping the controls constant, you can be sure that any differences in the results are due to the variables that you are testing.
  • The variables that you will test: The variables that you test are the elements of your experiment that you manipulate to test your hypothesis. Each variable must be carefully chosen to ensure that it will have a significant impact on the outcome of the experiment. It is also important to control for the effects of other variables.

By considering these key elements, you can create an effective experimental design that will allow you to answer your research question.

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Assignment Task 2: Design and critique experimental strategies to address biochemical questions.

Many different experimental strategies can be used to address biochemical questions. Some common approaches include:

  1. In vitro experiments: In vitro experiments are conducted using isolated cells or molecules in a controlled environment. This type of experiment is often used to study the effects of specific chemicals or mutations on biochemical processes.
  2. In vivo experiments: In vivo experiments are conducted using living organisms. This type of experiment is often used to study the effects of drugs or mutations on whole cells or organisms.
  3. Genetic engineering: Genetic engineering is a technique that allows scientists to edit the DNA of cells. This technique can be used to study the effects of specific genes on biochemical processes.
  4. Animal models: Animal models are often used to study the effects of mutations or drugs on biochemical processes. These models can be used to study the effects of diseases on humans.
  5. Clinical trials: Clinical trials are conducted on human patients. This type of study is used to test the safety and efficacy of new drugs or treatments.

Each of these experimental strategies has its advantages and disadvantages. It is important to choose the right strategy for your specific research question.

Assignment Task 3: Perform basic statistical analyses of experimental outputs.

Once you have collected your data, it is important to analyze it to see if your hypothesis was supported. Many different types of statistical tests can be used for this purpose. Some common tests include:

T-tests: T-tests are used to compare the means of two groups of data. This type of test is often used to determine the significance of differences between experimental and control groups.

ANOVA: ANOVA is used to compare the means of more than two groups of data. This test is often used to determine the significance of differences between multiple experimental groups.

Chi-squared tests: Chi-squared tests are used to determine the statistical significance of differences in frequencies between two groups of data. This test is often used to compare the frequencies of different genotypes in a population.

Once you have chosen the appropriate statistical test for your data, you will need to calculate the p-value. The p-value is a measure of the significance of the results of your experiment. A p-value less than 0.05 indicates that the results are statistically significant.

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Assignment Task 4: Describe ethical principles and good research conduct.

The ethical principles of good research conduct are designed to protect the welfare of research participants. Some key principles include:

  • Informed consent: Informed consent is the process by which research participants are given information about the risks and benefits of participating in a study. This process allows participants to make an informed decision about whether or not to participate in the study.
  • Confidentiality: Confidentiality is the process of protecting the identity of research participants. This process ensures that participants’ personal information is not shared with anyone outside of the study.
  • Respect for autonomy: Respect for autonomy is the principle that research participants have the right to make their own decisions about whether or not to participate in a study.
  • Beneficence: Beneficence is the principle that research should be designed to benefit participants. This principle is often used to justify the use of placebo-controlled trials.

Assignment Task 5: Demonstrate knowledge of core biochemistry, molecular biology, cell biology and genetics principles.

The core principles of biochemistry, molecular biology, cell biology, and genetics are essential for understanding the workings of cells and organisms.

Core biochemistry principles

  • The chemical structure of DNA and RNA: DNA is a double-stranded molecule that consists of two strands of nucleotides. RNA is a single-stranded molecule that consists of a sequence of nucleotides.
  • The role of enzymes in biochemical reactions: Enzymes are proteins that catalyze biochemical reactions. Enzymes can be found in all cells and play a vital role in metabolism.
  • The role of proteins in cell function: Proteins are the building blocks of cells. They are responsible for carrying out the essential functions of cells.
  • The role of carbohydrates in cell function: Carbohydrates are essential for the structure and function of cells. They are necessary for the cell to maintain its shape and to provide energy for cellular processes.
  • The role of lipids in cell function: Lipids are essential for the structure and function of cells. They play a vital role in cell membranes and are necessary for the cell to maintain its shape.

Molecular biology principles

  • The structure and function of genes: Genes are the units of inheritance. They are made up of DNA and are responsible for encoding the information needed to produce proteins.
  • The role of DNA in gene expression: DNA is responsible for the expression of genes. It is the template from which RNA is synthesized, and RNA is responsible for the translation of genes into proteins.
  • The role of RNA in gene expression: RNA is responsible for the translation of genes into proteins. RNA is synthesized from DNA, and proteins are synthesized from RNA.
  • The role of proteins in gene expression: Proteins are responsible for the regulation of gene expression. They bind to DNA and control the rate at which genes are expressed.

Cell biology principles

  • The structure and function of cells: Cells are the basic unit of life. They are made up of a membrane, cytoplasm, and DNA.
  • The role of cell membranes in cell function: Cell membranes are responsible for the selective permeability of cells. They control what enters and exits the cell.
  • The role of cell organelles in cell function: Cell organelles are responsible for the metabolism and function of cells. They are necessary for the cell to carry out its essential functions.
  • The process of cell division: Cell division is the process by which cells reproduce. Cells divide into two daughter cells, each of which has the same amount of DNA as the parent cell.

Genetics principles

  • The structure and function of chromosomes: Chromosomes are the structures that carry the genetic information of an organism. They are made up of DNA and proteins.
  • The role of DNA in inheritance: DNA is responsible for the inheritance of genetic information. It is the molecule that encodes the information needed to produce proteins.
  • Mutations and their effects on gene expression: Mutations are changes in the DNA that can lead to changes in the proteins that are produced. Mutations can be caused by errors in replication or by exposure to environmental factors.
  • The use of genetic engineering techniques in biomedical research: Genetic engineering is the process of manipulating the DNA of an organism. It is used to study the function of genes and to produce proteins for use in biomedical research.

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Assignment Task 6: Accurately use biochemistry terminology and nomenclature.

There is a lot of biochemistry terminology and nomenclature that you will need to know. Below are some key terms and their definitions that you should familiarize yourself with.

  • Amino acid: An amino acid is a molecule that contains both an amine group and a carboxylic acid group. Amino acids are the building blocks of proteins.
  • ATP: ATP is a molecule that stores energy in the form of phosphate bonds. ATP is used to power cellular processes.
  • Carbohydrate: A carbohydrate is a molecule composed of carbon, hydrogen, and oxygen. Carbohydrates are essential for the structure and function of cells.
  • DNA: DNA is a molecule composed of deoxyribose nucleotides. DNA is responsible for the expression of genes.
  • Enzyme: An enzyme is a protein that catalyzes chemical reactions in the body. Enzymes are essential for the metabolism and function of cells.
  • Gene: A gene is a unit of inheritance. It is made up of DNA and is responsible for encoding the information needed to produce proteins.
  • RNA: RNA is a molecule composed of ribose nucleotides. RNA is synthesized from DNA and is responsible for the translation of genes into proteins.
  • Protein: A protein is a molecule composed of amino acids. Proteins are essential for the structure and function of cells.

These are just a few of the many terms that you will need to know as a student of biochemistry.

Assignment Task 7: Perform biochemical calculations with accurate use of units.

To do biochemical calculations, you will need to be familiar with the units that are used in biochemistry. Some of the most common units are below.

  • Mole: A mole is a unit of measurement that is used in chemistry. It is equal to 6.022 x 1023 particles.
  • Atom: An atom is the smallest particle of an element that has the chemical properties of that element.
  • Molecular weight: The molecular weight of a molecule is the sum of the atomic weights of the atoms in the molecule.
  • Percentage: A percentage is a unit of measurement that is used to express the relative amount of one substance in another. It is expressed as a number between 0 and 100.

Many other units are used in biochemistry, but the ones listed here are some of the most common. Be sure to familiarize yourself with them so that you can do biochemical calculations accurately.

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